CRISPR-Test Assay Kits 12 nt Unique Dual Indices

12 nt Unique Dual Indices (UDIs) included

The bias is NOT introduced when using the “all samples” or “all assays” plate layout or when performing ΔΔCq based analysis

The primers have been designed to be exon-spanning where possible

ValidPrime® Vertebrate does not include a gDNA standard

Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube

CRISPR-Test Assay Kits 12 nt Unique Dual IndicesAccurately Assess Cas9 Activity in Cells Measure functional activity of Cas9 nuclease, dCas9 repressor, or dCas9 activator in any cell line Obtain quantitative FACS based read outs Get complete, easy to use kits with ready to transduce lentiviral particles The CRISPR Test assay kits contain a mix of lentiviral particles with both green fluorescent and red fluorescent protein (GFP and RFP). The GFP fluorescent marker responds to the specific Cas9