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Prepare working dilution by adding 5 μL PC to 45 μL Buffer A and mix thoroughly (pipette mixing
5 × 106 cells/mL Format Cryopreserved Vial Recommended Split Ratio 1:2 Typical Expansion 3–5 passages under the specified culture conditions Recommended Surface Gelatin pre-coated tissue culture flask or plate Recommended Incubation 37 °C in a humidified incubator with 5% CO2 Characterization Markers E-cadherin and ZO-1 by immunofluorescence staining Contamination Testing Negative for mycoplasma
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significantly enhancing functional integration of neural repair grafts with host neural circuitry
Swissprot No O75751 Gene Accession NP_068812 WB Predicted band size 61 kDa WB Positive control WB Recommended dilution 500-2000 IHC predicted cell location Predicted cell location: Cytoplasm and Cell membrane IHC positive control Positive control: Human tonsil IHC Recommed dilution Recommended dilution: 50-200 Storage
GFP Expressing C57BL/6 Mouse Primary Cardiac Microvascular Endothelial Cells Size:Frozen Vial (1 x 10^6 cells) Prepare working dilution by addingDescription GFP Expressing Mouse Primary Cardiac Microvascular Endothelial Cells from Cell Biologics are isolated from heart tissue of pathogen free laboratory C57BL 6 mice and grown in gelatin pre coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo preserved in vials. Each vial contains 0. 5x106 cells per ml and is delivered frozen. GFP Expressing Mouse Primary Cardiac
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