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Add 30uL paraffin oil if heat block
ED0077 (25g)
The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃
autosampler injection
The spots are counted manually under microscope or by automatic ELISPOT analyzer
RIPA Lysis Buffer IIII with EDTA and EGTA (pH 7.4) 2 x concentrate- 500ML Package Size:500mL Add 30uL paraffin oil ifDescription 2 x RIPA Buffer IIII with EDTA and EGTA (pH 7. 4) 2x concentrate: RIPA buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins and is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification. Extraction
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