Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Pantothenate Size:1000g Both Folic acid and Riboflavin

Both Folic acid and Riboflavin are omitted from the medium to make it suitable for fluorescence measurements with yeast cultures

3 mM 20 g/L EDTA Na2 20

Store at room temperature - Keep away from light

resulting in inhibition of both transpeptidation and translocation reactions

4% stock solution in ethanol) of 9 ml per litre medium has to be added prior to autoclavation

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Pantothenate Size:1000g Both Folic acid and RiboflavinCalcium pantothenate is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium pantothenate or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store