Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Manganese Size:1000g FM Amino acids drop-out mixtures

FM Amino acids drop-out mixtures are used in combination with FM Minimal medium w/o Amino acids or FM Minimal medium w/o Amino acids and w/o Ammonium chloride to complete the medium

Usage above a 20 mM concentration in mammalian cell culture work is not recommended

Peptone 2 Yeast Extract 0

SIH Amino acids drop-out mixtures are used in combination with SIH Minimal medium w/o Amino acids or SIH Minimal medium w/o Amino acids and w/o Ammonium chloride to complete the medium

1% Heavy Metals <0

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Manganese Size:1000g FM Amino acids drop-out mixturesManganese sulphate, MnSO4. H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Manganese or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store