Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Zinc Size:100g Tryptone 10 NaCl 8 Agar

Tryptone 10 NaCl 8 Agar 15 Final pH 7

10 Absorbance 280 nm <0

Improvement of a synthetic medium for Dictyostelium discoideum

05  M  30

Weight 112

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Zinc Size:100g Tryptone 10 NaCl 8 AgarZinc sulphate, ZnSO4. 7H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Zinc or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room